As AIDs was pervading in the worldwide, the research and development of medicine possessing anti-HIV function became more and more important. Several nucleoside medicine approved by FDA revealed great advantage and essentiality.

    5-methyluridine is the important intermediate in the chemical synthesis of anti-AIDS novel pharmacy---azidothymidine(AZT) or 2',3'-didehydro-2',3'-dideoxy-thymidime(d4T). Preveniently 5-MUR was chemical synthesized from inosine or tetraacetylribose and the production cost is about 150$/kg. However, if 5-MUR was synthesized by enzyme, the cost would reduced to $75/Kg, and there was no organic  solvent demanded or no environment pollution.

    If enzymatic synthesis of 5-methyluridine was successful, the price of AZT and d4T would be declined greatly. It would be much benefited to all HIV sufferer.

    5-fluorouridine(5-FUR) is the intermediate of flutulon and mainly synthesized from uridine. In the chemical process, the cost of 5-FUR is above $1500/kg and the final cost of Furtulon was above $8000/Kg if the price of uridine is about $150/kg. But if FUR was synthesized with enzyme, any  cheapness nucleoside could be used as raw material and through one step of reaction FUR could be obtained. For example, compare with chemical synthesis, cost of Futulon would be reduced 60% if guanosine($30/Kg) was used.

    There was low cost, high yield and little pollution in the enzymatic synthesis of 5-FUR. This method was the research direction abroad. As far as know, the conversion yield of 5-FUR is only about 19% and the technique had no practicality. However in our laboratory, the conversion yield reached to 60% and the method had been applied patent.

    As the same principle, if 5'-deoxyinosine or 5'-deoxyguanosien could be chemical synthesized, Futulon maybe enzymatically synthesized directly. The cost of production could decline to below $600/Kg. This techniques had been successed in development by our lab. We starved for investment to practise.

   1。Enzymatic synthesis of ribavirin

      The production cost would decline to below $60/Kg if guanosine or inosine was used as raw material. The conversion yield could reach above 85%.

   2。Enzymatic synthesis of 2'-deoxy-5-fluorouridine

      The conversion yield could arrive at above 50% if 2'-deoxyuridine and 5-FU were used.

   3。Enzymatic synthesis of thymidine

     The production cost of thymidine maybe only $200/kg if 2'-deoxyinosine was chemically synthesized as raw material and the conversion yield could achive above 50%.

   4。Enzymatic synthesis of 2'-deoxynucleosides

     The technique of chemical synthesis of thymidine had been industrialized. Thus if thymidine was used as raw material to enzymatically synthesize other nucleosides, the production cost would be only about $1000/Kg.