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Introduction of 5'-Nucleotides Item

　

 Name of Item:  100T/year of four 5’-Nucleotides

  5'-Nucleotides can be produced from Ribonucleic Acid. RNA was enzymatic degraded and nucleotides was seperated from the mixture. High purified 5'-AMP, 5'-GMP, 5'-CMP and 5'-UMP were recoveried.

　

 Introduction of Products

Nucleotides were mainly used as intermediate of medicine, functional food and food additive. Biochemistry medicine, such as ATP, cAMP and S-adenosylmethionine, could be produced from 5'-AMP. 5’-GMPNa2 has special 香菇flavor and can be used to produce especial delicious ajinomoto and kinds of seasonings. Cytidine diphosphate choline, CTP, Ara-C and polyinosylcytidine can be prepared from 5’-CMP. 5’-UMP,which has important physiological function, takes part in biosynthesis of glucuronide, a detoxifcation compound in liver. Also, it can be used to produce UTP, polyadenosyluridne, UDP-glucose,and ddI. Recently nucleotides were added to baby milk powder in Europe, in America and in Japan. It's reported that it possibly was beneficial to baby's immunity. Therefore nucleotides, mixed with single high refined nucleotide according to some ratio, could be used to functional foods.

 

Explanation of Technique

There's many virtue in the technique, such as high technique, new process, high yield, low cost, good quality and little pollution. The key technology of seperation had been applied to patent and authenticated by Shangdong Technology Committee. The process was original in the state. The charateristic could be summarized as follow three items.

1．The cost of enzyme and investment decreased 10-15% through improvement on enzyme producing and enzyme catalyze reaction. There was no waste water released in the process of enzyme producing. Conversion yield increased 10-15%. Side reaction was reduced. So basis was established to obtaine high purified nucleotides.

2．High purified nucleotides could be once recoveried through seriation combination of new ion exchange resin. The effusion from column was concentrated and crystallized. The quality of products could attain to above 98%.

3．New process resolved lots of disfigurement of old process in state, such as low conversion yield, low harvest, high cost and difficulty to scale-up production. The level of technology and the quality of products achieved advanced internationally.

 

 

Market Analysis

Enzymatic production of 5'-nucleotides from RNA is the primary process. The products and technique of 5'-nucleotides were cornered by Japan which has quantity of 5 kiloton/year. A little was produced in China. As recent years pharmacy of nucleic acid and Function food growed and springed up, Nucleotides shorted up a lot. AMP, CMP and UMP, not exported in Japan, were used to produce nucleic acid pharmacy except that IMP and GMP were exported as food additives. Not only 5'-nucleotides were demanded great nationally, but also developed countries in America and Europe expected to obtain nucleotides from China. Output and demand of four 5'-nucleotides were reviewed as follow:

1．      AMP：22 ton/year. AMP was mainly used to prepare ATP and cAMP. ATP,chemistry name as adenosine triphosphate, a high energy compound in organism, was intituled as currency energy coin in cells, and could be used as pharmacy to cure or assistant cure sweeny atrophy, block of miocardial infarction, hepatitis and other first-aid disease. Recently ATP alse was used as healthy food to increase level of body's energy in athlete or pharmacy to prevent block of miocardial infarction in old men. Very year 50T ATP had to be imported in our country and millions of foreign exchange had to be used. So AMP becamed a kind of short-up product and its price increased to $300/kg from $100/Kg.

2．      GMP：30ton/year. As food additives, GMP were mainly imported to our country. Every year flavor nucleotides, 200 ton IMP+GMP were imported from Japan.

3．      CMP：18ton/year. CMP were mainly used to produce CDP-C, Polyinosylcytidine and CTP. Although the technique of production CDP-C was matured, every year 20ton of CMP had to be imported because of lack.

4．      UMP：30ton/year。The product could be used to produce many kinds of pharmacy. UMP were mainly exported.

 

Cost checking and Economic analysis

The mail material's cost is RNA. 200t/year RNA were needed according to 50% yield. According to its top price of ￥200/kg, the cost of RNA was $5,000,000/year. The whole cost of water, electricity, steam, enzyme, acid and alkali was less than $1,200,000/year. If average price of four nucleotides was calculated as $120/Kg, the whole production value of 100T/year would reach to $10,000,000/year.

The tax was estimated to $5,000,000/year.（40%）。

 

Risk analysis

Currency technique was matured and authenticated by Shandong Technology Committee. We succeeded in scale-up producing in Shangdong province. The products of nucleotides prepared by us had been exported to Europe and  approbated by foreign merchants. The amout of export was estimated to 50T/year and would increas in future. 100T/year nucleotides were needed in the state. So the market foreground of Nucleotides was very good. The investment of whole project was about $4,000,000. The production value would be $10 million. The disinvestment would be complete in 1-2 year.

The main material is RNA, but about 400T/year were prduced in our country. The material was no problem because there was not a factory that could produce large amount of nucleotides.

 

Standard of nucleotides

  Description: white crystalline powder

　

Structure of four nucleotides:

AMPNa2	GMPNa2	CMPNa2	UMPNa2

                                                                                          

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